No our 3 x 6 glass subway tile comes aligned on the mesh sheet. We do this to reduce the risk of damage on shipping. But don't worry if you want to installed it in a staggered or brick pattern you can easily slice the mesh and install the tile 2 at a time.
Here is a video showing this exact process.
http://www.subwaytileoutlet.com/Glass-Subway-Tile-Videos.html